Signalling

Part:BBa_K511811:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Notch-Gal4-ESN Juxtacrine Receptor Generator (CMV-Notch-Gal4-ESN) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
    Illegal NotI site found at 5847
    Illegal NotI site found at 5855
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal BamHI site found at 3556
    Illegal XhoI site found at 6138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 3259
    Illegal NgoMIV site found at 3315
    Illegal NgoMIV site found at 3358
    Illegal NgoMIV site found at 4143
    Illegal NgoMIV site found at 5160
    Illegal NgoMIV site found at 5337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6057
    Illegal BsaI.rc site found at 716
    Illegal BsaI.rc site found at 2424
    Illegal BsaI.rc site found at 3339
    Illegal BsaI.rc site found at 5796


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References